The technique of introducing new genetic material into the germline of mammals has been a major development in biotechnology over the last decades."Transgenic animals" are animals whose chromosomes contain stable, integrated copies of exogenous genes, additional copies of endogenous genes or gene constructs. They are frequently created by two different techniques: microinjection of DNA into the pronucleus of zygotes and injection of embryonic stem cells into blastocysts. This video covers microinjection of DNA into the pronucleus of zygotes.
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